Özet:

The study aims to investigate the cytotoxicity on the human dermal fibroblast primary cells in order to prove the antibacterial activity and reliability of narcotic leather extract. The antibacterial effectiveness of ethanol extract from the nar skin was tested with disc diffusion and microdilusion methods against Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus MRSA ATCC 67101, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Enterococcus faecalis, Staphylococcus epidermidis, Burkholderia cepacia, Bacillus cereus, Citrobacter freundii and Cedecea net. At the same time, the effects of the extract on human dermal fibroblast cells were also determined by the WST-8 experiment. According to the results of the study, the ethanol extract of the nar skin, A. baumannii, E. coli, S. aureus MRSA ATCC 67101, P. aeruginosa, E. faecalis, S. epidermidis, B. cepacia, B. cereus, C. freundii and C. neteri were potentially influenced by the MIC values in proportions ranging from 100 to 500 μg mL-1. In the study, no inhibitor activity of the extract against K. pneumoniae, S. aureus and S. pneumoniae was observed. At the same time, Punica granatum L. skin ethanol extract had no cytotoxicity against skin fibroblast cells. According to the results of the study, it is recommended that the extract may also be used as a natural protective ingredient in many industrial products because it has antibacterial properties and has no toxic effect.

Anahtar Kelimeler:

Özet:

In this study, it was aimed to investigate the antibacterial activity of pomegranate peel’s extract and the cytotoxicity on the human dermal fibroblast primary cells to rationalize the safe usage of this extract. The antibacterial efficiency of ethanol extract of pomegranate peels was evaluated against Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus MRSA ATCC 67101, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Enterococcus faecalis, Staphylococcus epidermidis, Burkholderia cepacia, Bacillus cereus, Citrobacter freundii and Cedecea neteri by disc diffusion and microdilution assays. At the same time, the effects of this extract on the human dermal fibroblast primary cells were determined by WST-8 assay. The ethanol extract of pomegranate peels was potentially effective with different efficiency against A. baumannii, E. coli, S. aureus MRSA ATCC 67101, P. aeruginosa, E. faecalis, S. epidermidis, B. cepacia, B. cereus, C. freundii, and C. neteri at MIC's ranged from 100 to 500 μg mL-1. However, it exhibits no inhibition activity against K. pneumoniae, S. aureus and S. pneumoniae. The ethanol extract of Punica granatum L. peel exhibited no cytotoxic activity against the normal human dermal fibroblast primary cells. According to results it may be suggested that this extract possess antibacterial properties and the safety, and therefore, it can be used as a natural preservative ingredient in many industrial products.