Abstract:

Hypodermosis is a distinctive ectoparasitic disease infesting cattle; Hypoderma bovis and Hypoderma lineatum are the most common causes of this myasis. In this study 78 larvae were collected from infected cattle by extraction in the Educational Veterinary Hospital, from Kokjali and Bazwaya flocks and from the skin of slaughtered in Mosul abattoirs for the period from October 2020 to March 2021. Morphological identification by using stereomicroscope depending on patterns of spinulation of the 10th abdominal segment and peritremes structure of L3 classified as H. bovis and H. lineatum. Molecular technique by traditional PCR applied on 16 L3 of the genus Hypoderma revealed that the reaction product was 500 bp by amplification of mt CO1 gene while the results of PCR-RFLP using restriction TaqI enzyme for differentiation between the two species indicated reaction products 300bp for H. bovis and 200bp for H. lineatum respectively. The results of molecular analysis by PCR and PCR-RFLP proved the existence of these two species of Hypoderma in Mosul. 

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