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Şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisi
2013
Journal:  
Cumhuriyet Tıp Dergisi
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Abstract:

Özet Amaç. Çalışmamızda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisini tespit etmek amaçlanmıştır. Yöntem. Erzincan Bahçe Bitkileri Araştırma Enstitüsü Gen Bahçesi’nde bulunan Cardinal çeşidi 7 adet şeftali (Persica vulgaris Miller) ağacı yaprağı methanol ekstraktı bu amaçla kullanılmıştır. Antibakteriyel etkisinin araştırılmasında cut plug yöntemi kullanılırken Minimum İnhibisyon Konsantrasyonu (MİK), mikrodilüsyon yöntemi ile belirlenmiştir. Bulgular. Bu çalışmada şeftali (Persica vulgaris Miller) yaprak ektraktının Staphylococcus aureus, Staphylococcus aureus (MRSA)’a karşı antibakteriyel etkisi belirlenemezken, Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus ,Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica bakterilerine karşı ise antibakteriyel etki tespit edilmiştir. En düşük Minimum İnhibisyon Konsantrasyonu Pseudomonas aeruginosa ve Yersinia enterocolitica’da tespit edilirken (0,01 mg/mL),yüksek konsantrasyon Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes’de görülmüştür (10 mg/mL). Sonuç. Çalışmamız sonucunda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etki spektrumu invitro olarak tespit edilmiş olup bu tür bitkilerden yola çıkılarak yeni antibakteriyel ajanların oluşum sürecinin başlatılabileceği düşünülmektedir. Anahtar sözcükler: Persica vulgaris Miller, antibakteriyel etki, cut plug metot, methanol ekstraktı.   Abstract Aim. In our study we aimed to determine antibacterial effect of peach leaves extract. Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach’s (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. Results. In this study peach’s (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0.01 mg/mL), the higest concentration on grup of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). Conclusions. As a result of our study peach’s (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents. Keywords: Persica vulgaris Miller, antibacteriel effect, cut plug methot, methanol extract

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The antibacterial effect of Persica vulgaris Miller
2013
Author:  
Abstract:

The purposes. Our study aims to identify the antibacterial effect of peanut (Persica vulgaris Miller) leaf extract. The Method. The Cardinal species located in the Gene Garden of the Erzincan Garden Plant Research Institute has been used for this purpose 7 pieces of pepper (Persica vulgaris Miller) tree leaf methanol extract. In the study of the antibacterial effect, the cut plug method is used while the Minimum Inhibition Concentration (MIC) is determined by the microdilusion method. The findings. In this study, the antibacterial effect of sheet (Persica vulgaris Miller) leaf extract against Staphylococcus aureus, Staphylococcus aureus (MRSA) is uncoverable, while Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus ,Pseudomonas aeruginosa, Bacillus cereillus, Bacillus subtilis, Yoliters enterocolithic bacteria are antibacterial. The lowest minimum inhibition concentration was detected in Pseudomonas aeruginosa and Yersinia enterocolitica (0,01 mg/mL), while the high concentration was detected in Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes (10 mg/mL). The result. As a result of our study, the antibacterial effect spectrum of the Persica vulgaris Miller leaf extract has been identified as invitro, and it is believed that the formation of new antibacterial agents can be initiated from these plants. Keywords: Persica vulgaris Miller, antibacterial effect, cut plug method, methanol extract.   The Abstract. In our study we aimed to determine the antibacterial effect of peach leaves extract. The Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach's (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. The results. In this study peach's (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereilis, Bacillus subtolitica, Yocolitica enterolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0. 01 mg/mL), the higest concentration on group of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). and conclusions. As a result of our study peach's (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents. Keywords: Persica vulgaris Miller, antibacterial effect, cut plug methot, methanol extract

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Cumhuriyet Tıp Dergisi

Field :   Sağlık Bilimleri

Journal Type :   Uluslararası

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