User Guide
Why can I only view 3 results?
You can also view all results when you are connected from the network of member institutions only. For non-member institutions, we are opening a 1-month free trial version if institution officials apply.
So many results that aren't mine?
References in many bibliographies are sometimes referred to as "Surname, I", so the citations of academics whose Surname and initials are the same may occasionally interfere. This problem is often the case with citation indexes all over the world.
How can I see only citations to my article?
After searching the name of your article, you can see the references to the article you selected as soon as you click on the details section.
 ASOS INDEKS
 Views 11
Development of leptospiral outer membrane protein-based dot-ELISA assays and immunoprofiling for diagnosis of acute canine leptospirosis
2017
Journal:  
Turkish Journal of Veterinary and Animal Sciences
Author:  
Abstract:

The present study was carried out to evaluate outer membrane protein (OMP)-based relatively simple dot-ELISA assays for the diagnosis of acute canine leptospirosis and to identify the differential expression of leptospiral OMPs for identifying the markers of acute infection, if any. Among 91 canine serum samples screened, 63 samples were found to be positive by MAT. All the samples were tested by OMP-based IgM and IgG dot ELISAs. The sensitivities of IgM and IgG dot ELISA in comparison to MAT (titer: ≥1:400) were found to be 100%, whereas the specificities were found to be 89.71% and 45.31%, respectively. IgM and IgG immunoblotting of OMPs was standardized and tested with the MAT-positive samples. IgM immunoblotting of leptospiral OMP revealed proteins of 18, 21, and 32 kDa as major reactors. With IgG, proteins of 32, 41, and 66 kDa were recognized as major reactors and an 86-kDa protein was identified as a minor reactor. The 32-kDa protein was the predominant reactor in both IgM and IgG immunoblots. Thus, a combination of 18-, 21-, and 32-kDa proteins could be a good choice for the detection of IgM antibodies indicative of acute infection while that of 32-, 41-, and 66-kDa proteins could be employed for seroepidemiological studies.

Keywords:

Citation Owners
Information: There is no ciation to this publication.
Similar Articles










Turkish Journal of Veterinary and Animal Sciences

Journal Type :   other

Turkish Journal of Veterinary and Animal Sciences