Abstract:

The development of the fluorescent microscope is revolutionized with the invention of laser scanning confocal microscopy. With its three-dimensional display and analysis techniques, this technology has provided a more real view of the medical / biomedical world. Confocal microscope is based on a light microscopic technique for imaging fluorescently labeled samples with a three-dimensional structure. Confocal microscope applications include imaging the spatial distribution of macromolecules in fixed or live cells, collecting three-dimensional data, displaying multiple fluorescently labeled samples, and measuring physiological events in living cells. In this review; the principles of the laser scanning confocal imaging, history of confocal microskopy, features distinguished from other microscopes, as well as its innovative and important aspects and applications to medicine are presented.

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