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Kan kültüründe kalite yönetim sisteminin önemi: Kontaminasyon oranları
2021
Journal:  
Turkish Journal of Clinics and Laboratory
Author:  
Abstract:

Amaç: Kan dolaşımı enfeksiyonlarının tanısı, klinik mikrobiyoloji laboratuvarlarının en acil ve önemli görevlerinden biridir. Kontaminasyonu en aza indirerek doğru etkenin saptanması morbidite ve mortaliteyi doğrudan etkilemektedir. Bu çalışmamızda, kan kültürlerinde kontaminasyona neden olan preanalitik etkenlerin belirlenmesi amaçlanmıştır. Gereç ve Yöntemler: * Mikrobiyoloji Laboratuvarına 17.05.2017-08.11.2019 tarihleri arasında gelen örnekler otomatize kan kültürüne ekildi. Üreme sinyali veren örnekler boyama sonrası bakteriyel kültür yapılarak otomatize sistemle identifikasyon ve antibiyograma alındı. Sonuçlar kan dolaşım yolu enfeksiyonuna neden olduğu bilinen etkenler ve kontaminantlar açısından analiz edildi. Bulgular: Toplam 5215 kan kültür örneğinin 821 (%15,7)’nde üreme saptandı. Örneklerin 425 (%8,15)’i kontaminant olarak rapor edildi. Kontaminasyon oranı kadınlarda %8,7 erkeklerde %7,8 idi. Yaş gruplarına göre kıyaslandığında oran 18 yaş üstü grupta en yüksek (%9,3) iken 5-18 yaş grubunda en düşüktü (%3,4). Servis olarak Yoğun Bakımlarda kontaminasyon oranının en fazla (%13,8) olduğu görüldü. Sonuç: Kontaminasyon kanda organizma olmadığı halde kültürde üreme olması durumudur ve en önemli nedeni, cilt florasında bulunan mikroorganizmaların kan kültürü şişelerine inokülasyonudur. Hastane ortamı, kateteri kolonize eden mikroorganizmalar, kanı alan personelin elleri ve kültür alımında kullanılan ekipmanlar da kontaminasyon kaynağı olabilir. Bizim çalışmamızda kontaminasyon oranlarını yüksekti ve yaş grupları ile servisler arasındaki fark anlamlı bulduk. Kontaminasyon oranlarının düşürülmesi için kan eğitimli bir sağlık personeli tarafından alınmalı, etkin bir cilt antisepsisi uygulanmalı ve intravenöz kataterden örnek alınmamalıdır.

Keywords:

The importance of the lithium management system in the blood culture: rates of contamination
2021
Author:  
Abstract:

Diagnosis of blood circulation infections is one of the most urgent and important tasks of clinical microbiology laboratories. Identifying the correct factor by minimizing contamination directly affects morbidity and mortality. This study aims to identify the pre-analytic factors that cause contamination in blood cultures. Tools and Methods: * Examples from 17.05.2017-08.11.2019 to the Laboratory of Microbiology were automatically planted into the blood culture. Examples that give reproductive signals were taken with the automated system of identification and antibiotics by making bacterial culture after painting. The results were analyzed in terms of known factors and contaminants that caused blood circulation infection. Results: Reproduction was found in 821 (15.7%) of a total of 5215 blood culture samples. 425 of the samples (8.15%) were as contaminant. The rate of pollution was 8.7% in women and 7.8% in men. Compared to age groups, the rate was the highest in the age group over 18 years (9.3%), while the lowest in the age group 5-18 years (3.4%). As a service, the highest rate of contamination in intensive care was found (13.8%). The result: Contamination is a condition of reproduction in the culture while there is no organism in the blood and the most important cause is the inoculation of microorganisms in the blood culture bottles in the skin flora. The hospital environment, the microorganisms that colonize cathetes, the hands of the blood receiving staff and the equipment used in the culture acquisition can also be a source of contamination. In our study, the rates of contamination increased and we found the difference between age groups and services meaningful. To reduce the rates of contamination, the blood should be taken by a trained health staff, an effective skin antiseptic should be applied and the intravenous cataracts should not be taken.

Keywords:

Importance Of Quality Management System In Blood Culture: Contamination Rates
2021
Author:  
Abstract:

Aim: Diagnosis of bloodstream infections is very important for microbiology. Determining the correct agent by minimizingcontamination directly affects morbidity and mortality. In this study, it was aimed to determine the preanalytical factors causing contamination in blood cultures. Material and Methods: The samples that sent to Microbiology Laboratory between 17.05.2017-08.11.2019 were added to automated blood culture. Reproductive signal samples were stained and bacterial cultures were made. Results: Bacterial growth was detected in 82 (15.7%) of 5215 culture samples. 425 (8.15%) of the samples were contaminants. This rate were 8.7% in women; 7.8% in men. In age groups, the ratio was highest above the age of 18(9.3%); It was the lowest at the age of 5-18(3.4%). In services, the rate of contamination in intensive care units was highest (13.8%). Conclusion: Contamination is the growth in culture, although there is no organism in the blood. The most important reason is the inoculation of microorganisms in the skin flora into culture bottles. Hospital environment, microorganisms that colonize the catheter, the hands of the staff who take the blood and the equipment used to take culture can alsobe a source of contamination. In our study, contamination rates were high and we found the difference between age groups and services significant In order to reduce the contamination rates, blood should be taken by a trained healthcare professional, an effective skin antisepsis should be applied and sample should not be taken from the intravenous catheter.

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Turkish Journal of Clinics and Laboratory

Field :   Sağlık Bilimleri

Journal Type :   Uluslararası

Metrics
Article : 620
Cite : 334
2023 Impact : 0.019
Turkish Journal of Clinics and Laboratory