Özet:

Objective: There are some strong evidence about the role of nitric oxide (NO) as an intercellular messenger in the central physiological mechanisms. It is suggested that NO might be anticonvulsant, proconvulsant or ineffective on seizure activity in various experimental seizure models. In this study, the effects of 7-nitro indazole (7-NI), a selective inhibitor for the form of nitric oxide synthase enzyme found in the brain, on strychnine (ST) and pentylenetetrazole (PTZ) induced seizures in mice were investigated. Methods: ST (1.5 mg/kg) or PTZ (85 mg/kg) were administered subcutaneously (s.c.) to produce seizures. 7-NI, vehicle or saline (0. ı ml/25g, i.p.) were given 30 minutes prior to ST or PTZ to separate mice groups. 7-NI(30mg/kg) was dissolved in arachis oil (AO; peanut oil) for intraperitoneal (i.p.) administration. Results: The first myoclonic jerk (FMJ), the total convulsion time (TCT), the survival time, and the rate of mortality was recorded. 7-NI had no effect on all parameters, both in ST and PıZ induced seizures. Interestingly AO compared to saline controls significantly delayed FMJ and survival time; increased TCT; decreased the rate of mortality induced by ST (p < 0.05). AO also significantly delayed FMJ induced by PTZ compared to saline controls. Conclusion:  It is very possible that the i.p. injection of AO could have altered absorption and/or distribution of the subsequent s.c. injection of ST or PTZ. These observations suggested that 7-NI, at dose and time intervals used in this study, had no effect on seizure activity.

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