Abstract:

In this study, we aimed to investigate the protective role of bromelain against nickel sulfate genotoxication. Twenty-four healthy adult male Sprague Dawley rats with an average weight of 200 ± 20 g were used in the study. These were divided randomly into four groups (n = 6), including one control and three experimental groups: Group 1 (control), Group 2 (nickel sulfate 20 mg/kg, intraperitoneal [IP]), Group 3 (Bromelain 20 mg/kg, oral gavage ), Group 4 (nickel sulfate 20 mg/kg IP + bromelain 20 mg/kg oral gavage ). After the ten-day experimental period, the animals were euthanized on the 11th day. Femoral bones were taken from animals dissected on day 11, and micronucleus protocol was applied. At the end of the protocol, micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes (PCE) were counted. The statistical results of the counted values were determined. The groups were compared only according to MNCPE, the level of which was statistically significant in the group with bromelain than in the group with nickel sulfate alone (P < .05). The data obtained suggested that bromelain at the administered dose (20 mg/kg) is not potentially preventive to the genotoxic effects of nickel sulfate at the administered dose (20 mg/kg).