Özet:

Objective: Age-related-macular-degeneration (AMD) is a chronic disease characterized by vision loss caused by neurodegeneration of the retinal pigment epithelial complex. Excess reactive oxygen species(ROS) play an important role in the development of retinal diseases, especially macular degeneration. The main ROS are; peroxynitrides, superoxide radicals and hydrogen peroxides. In our study, we aimed to investigate the protective effect of before oxidative damage induced by hydrogen peroxide(H2O2) in cell culture. Materials and Methods: Oxidative stress was induced with H2O2 in human retinal pigment epithelial(ARPE-19) cells. Oxyresveratrol was applied at 7 different concentrations before oxidative damage. Protective effects were investigated by monitoring the change in cell viability with the XTT cell proliferation assay. Caspase-3 and cell-death-detection kit were used to investigate the protective effect of oxyresveratrol and its molecular mechanism. Results: In our study, oxyresveratrol application before oxidative damage induced by H2O2 in ARPE-19 cell line increased cell viability and showed protective activity against oxidative damage in the cell. In the findings we obtained as a result of our study; When oxyresveratrol was applied to ARPE-19 cells before the oxidative damage induced by H2O2, it reduced cell damage by about 15% at 100 µM concentration. In addition, according to cell death detection and caspase-3 results, oxyresveratrol showed protective activity against oxidative damage by reducing apoptotic cell death. Conclusion: This in vitro study is a preliminary study for the development of the protective effect of oxyresveratrol. Oxyresveratrol can be developed as an effective therapeutic agent in the prevention of retinal diseases, especially AMD, after experimental animals and clinical studies.

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