Abstract:

Purpose: Cystic equinococcus (KE) is one of the most common zoonotic infections in the world. The diagnosis of KE is mostly based on visualization techniques, and serological tests are used to help diagnose in cases where there are non-characteristic visualization findings. However, the results of serological tests are not fully reliable because they are affected by many factors. P-selectin and resistin are known as inflammatory indicators that change in the acute stages of infection. For this purpose, inflammatory indicators such as P-selectin and resistin have been studied as a potential diagnostic reference for KE diagnosis. Methods: In this study, a total of 60 patients diagnosed with KE and 25 healthy individuals were taken blood samples from vetum participants. The resulting serum has been evaluated using P-selectin and resistin ELISA kit for protein levels. The relative expression of SELP (P-selectin) and RETN (resistant) genes has also been determined by using the comparative CT (ΔΔCT) method between active and inactive cysts of KE patients and healthy controls. Results: SELP (13.9 times, p<0,05) and RETN (8.1 times, p<0,05), different levels of expression were detected in KE patients compared to the control group. While resistant protein levels in KE patients were significantly high compared to healthy controls (p<0,001), the difference between P-selectin protein levels was not significant (p>0,05). There was no difference between the levels of genes and proteins of active and inactive KE patients in terms of P-selectin and resistin (p>0,05). Result: As a result, although there is no difference between the gen and protein levels of inflammatory indicators in active and inactive KE patients, it has been shown that due to the good difference between healthy controls and KE patients, resistance may be a potential inflammatory diagnosis reference.

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