Abstract:

Background: Pancreatic cancer is among those with the worst prognosis of all cancers. The Tripartite Motif-Containing Protein 3 (TRIM3) gene, as a tumor suppressor gene, plays a role as a tumor suppressor by controlling the proliferation, migration and invasion of cancer cells. The aim of this study was to investigate the expression of TRIM3 gene at the mRNA level in AsPC1, BxPC-3 and PANC-1 pancreatic cancer cell lines. Materials and Methods: AsPC1, BxPC-3 and PANC-1 cell lines were cultured at 37˚C in an environment containing 5% CO2 and total RNA was isolated. TRIM3 gene mRNA expression level was analyzed by Quantitative Reverse Transcription PCR (RT-qPCR) method. Analysis of relative gene expression data was performed using the 2-∆∆CT method. Results: The mRNA expression levels of TRIM3 were found to be very low in all three cell lines. In addition, when fold change was calculated, no difference was observed between cell lines. Conclusions: TRIM3 gene plays a role as a tumor suppressor gene in the process of carcinogenesis and it has been shown that TRIM3 expression is decreased in cancer cells. Consistent with other cancer types in the literature, TRIM3 mRNA expression was found to be very low in pancreatic cancer cells. Since this study is the only study investigating the relationship between AsPC1, BxPC-3 and PANC-1 pancreatic cancer cell lines and TRIM3, it will shed light on future functional studies.

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