Abstract:

Purpose: In the framework of this study, the potential benefits of brusatol against over tissue damage caused by bilateral over tortion/detortion (T/D) were tried to be investigated. Tools and Methods: In this study, the experimental animals were randomized into 4 groups. The groups were programmed as Group I (sham-control), Group II (T/D), Group III (DMSO+T/D) and Group IV (Brusatol+T/D). The group I area of the abdomen was scratched, disinfected, opened with a cut and closed again, but the T/D model was not applied. In Group II, as described in Group I, 3 hours of tortion was applied after the abdominal injection and followed by 3 hours of detection. The group III, 1% DMSO, was administered intraperitoneally at 0.3 ml as daily for 10 days, and the last dose was administered 30 minutes before the detection. After that, all the processes were carried out as described in Group II. Group IV brusatol was administered intraperitoneally every 2 days for 10 days, and the last dose was given 30 minutes before detection. After the deforestation period, the rats were sacrificed and over tissues were removed. Results: Compared to Group I in Group II and III, the levels of oxidative parameters (MDA, MPO, TOS, OSI) and proinflammatory cytokine (TNF-α, IL-1β) increased, while the antioxidant (SOD, TAS) parameters significantly decreased (p<0.05). On the contrary, the application of brusatol (Group IV) reversed all of these values compared to Group II and III (p<0.05). Result: As a result, it was estimated that brusatol has protective effects against over damage caused by T/D in the famous rats.

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Abstract:

Objective: In the scope of this study, it was tried to examine the probable benefits of brusatol against ovarian tissue injury originating from bilateral ovarian torsion/detorsion (T/D). Material and Methods: In this study, experimental animals were randomized to 4 groups. Groups were programmed as sham group I (control), group II (T/D), group III (DMSO+T/D) and group IV (Brusatol+T/D). Group I; the abdomen region was barbered, disinfected, opened by incision and closed respectively, but no T/D model was performed. Group II, as described in group I, after opening and closing abdominal incision, 3 hours of torsion was applied and it was followed by a 3-hour of detorsion. Group III, 1% DMSO was applied intraperitoneally 0,3 ml as every other day for 10 days and the last dose was given 30 minutes prior to detorsion. Group IV; brusatol was administered intraperitoneally 0,5 mg/ml once every 2 days for 10 days and the last dose was applied 30 minutes prior to detorsion. Then, all processes were carried out as described in group II. Following detorsion period, in pursuit of sacrificing the rats, ovarian tissues were removed. Results: Oxidant parameters (MDA, MPO, TOS, OSI) and pro-inflammatory cytokine (TNF-α, IL-1β) levels increased significantly while antioxidant levels (SOD, TAS) reduced in group II and III compared to group I (p<0.05). On the contrary, brusatol application (group IV) reversed all of these values compared to group II and III (p<0.05). Conclusion: As a conclusion, brusatol was evaluated to have protective effects against T/D-induced ovarian injury in rats.