Abstract:

Adriamycin is a widely used chemotherapy drug in the treatment of cancer. Adriamycin has harmful effects on many tissues including testis as well as cancercells. Selenium and Vitamin E have reproductive organs and protective properties on infertility. The aim of this study to investigate the protective effect of Vitamin E and Selenium against Adriamycinin duced testicular injury. In this study, 64 male rats were 8 in each group; Control, Adriamycin, Vitamin E, Selenium, Vitamin E+Selenium, Adriamycin+Vitamin E, Adriamycin+Selenium, Adriamycin+VitaminE+Selenium divided in to groups. The testis tissues were excised and fixed in 10% formaldehyde and embedded in parafin blocks through routine histological follow-up steps. Sections of 5 µm thickness were stained with Hematoxylen&Eosin for histopathological evaluation and studied underlight microscope. The damage in the seminiferous tubules was determined through JTBS. Malondealdehyde, Superoxide Dismutase, Catalase and Glutathione Peroxidase activities were investigated by biochemical methods. Testosterone levels were measured from the serum samples. Apoptotic cells were counted by the TUNEL method. As a result, when the histopathological evaluation of the tissues was completed, irregularities were observed in the testicular tissues of the ADR group, unlike the other groups. According to the results of the TUNEL method, the number of TUNEL positive cells in the ADR group was increased compared to the other groups (p=0.001). When the values of MDA (p = 0.593), CAT (p = 0.469), GPX (p = 0.655) and SOD (p = 0.907) were compared in testicular tissue, nosignificant difference was found between the groups. However, testosterone levels were lower in all ADR groups compared to the other groups and this decrease was statistically significant. Testosterone levels were found to be lower in all ADR groups. In this context, there was a significant difference between the groups in terms of testosterone levels (p = 0.001). As a result, Adriamycin administration caused damage to rat testicular tissue. Vitamin E and Selenium treatment have proven to have a partial corrective effect on testicular tissues.

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