Özet:

The Verticillium Dahliae Cleb. Cotton and olive production is an important fungal disease that causes serious productivity and plant losses everywhere. It makes it difficult to fight the disease from the ground. The biological struggle in fungal diseases is on the forefront day by day, and micoviral dsRNAs have also begun to be used as a biological agent. There have been four studies on verticillium dahliae micoviral dsRNAs, one in cotton and one in olive. However, in Turkey, V. dahliae, which caused polio disease in the cotton plant, has not found a study on the presence of micovirus dsRNA. In this study, 72 V is obtained from 100 samples showing the signs of pollution from cotton areas of the Aydin or Söke and Koçarlı districts. The presence of micovirus dsRNA in the dahliae isolation has been studied and the dsRNA in the twelve V. dahliae isolation has been determined. The recognition of V.dahliae micoviral dsRNA with RT-PCR was made using specific primes and random hegzamer primes designed for Verticillium dahliae partitivirus 1-olive (VdPV1-ol) micovirus in olive oil. After all, these primates we use have been found that the dsRNA micovirus in the V.dahliae isolates in cotton cannot be recognized. In the study, an apple test was applied to determine the virulence of the V. dahliae isolates containing dsRNA but no result was obtained.

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