Abstract:

A simple, rapid, precise, accurate, economical and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of Chlorthalidone and Metoprolol Succinate in combined pharmaceutical formulations. The separation wasachieved on a Thermo Scientific BDS column (250 × 4.6 mm i. d, particle size of 5 µ) using a mobile phaseconsisting of Acetonitrile: 0.1% Trimethylamine (90:10 %v/v) (pH adjusted to 4.5with 0.1% orthophosphoric acid). The mobile phase was pumped at a flow rate of 1ml/min. The UV detection was monitored at 224nm. The retention times of Chlorthalidone and Metoprolol Succinate were found to be 2.6min and 6.5min respectively. Excellent linearity range was found between 4-14µg/ml for Chlorthalidone and 16-56µg/ml for Metoprolol Succinate. The method was validated with respect to linearity, precision, accuracy, specificity and ruggedness. Method was successfully applied for the simultaneous determination of Chlorthalidone and Metoprolol Succinate in combined pharmaceutical dosage form.

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