Abstract:

The purpose of this study was to determine the bioactive compounds of laurel oil and to evaluate the effect of different doses of laurel oil on rumen fermentation traits, gas formation and nutrient disappearance using in vitro rumen simulation technique. The trial was conducted with in vitro rumen by using Rusitec system and 2 treatments that is Laurel oil supplied at 50 mg/L and 100 mg/L. A basal diet consisted of a 0.48:0.52 ratio of hay and concentrate on a dry matter (DM) basis. The samples were analysed for fermentation parameters (pH, ammonia, volatile fatty acid (VFA), microorganism population and gas production) and the bioactive compounds of laurel oil and fermenter fluid samples were determined by gas chromatography (GC) and GC- (mass spectrophotometer) MS. Major constituents of the laurel oil was limonene (64.6%): Limonene did not affect the rumen fermentation, apparent nutrients degradation and methane emission. Total volatile fatty acid concentration was not affected but an increase was observed in isobutyrate proportion together with a quadratic influence in propionate proportion. Results showed that laurel oil supplementation did not affect negatively rumen fermentation and

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