Özet:

Purpose: Purpose of this study is to examine the effect of aprotinin on ischemia-reperfusion (IR) injury that occurs in kidneys after occlusion-reperfusion of infrarenal abdominal aorta (IAA) in rats. Methods: Twenty-four Wistar-Albino rats were randomised in equal numbers (n = 8) into three groups. Control group underwent laparotomy and IAA dissection but not occlusion. Aortic IR group underwent 30 min of ischemia by clamping of IAA and 60 min of reperfusion by declamping of IAA. Aortic IR + aprotinin group underwent aortic IR and received 40000 KIU/kg intravenous bolus of aprotinin 5 min before declamping. Tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase and glutation peroxidase were measured in rat kidney specimens. Histological examination of the kidney tissue specimens was also done. Results: Biochemical analysis showed that, aortic IR significantly increased (p<0.05 vs control) while aprotinin significantly decreased (p<0.05 vs aortic IR) the levels of MDA and SOD. There was no statisticaly significant difference between the levels of catalase and glutation peroxidase in the groups (p>0.05). Histological examination showed that aortic IR significantly increased (p<0.05 vs control) while aprotinin significantly decreased (p<0.05 vs aortic IR) the parameters of focal necrosis in glomerulus, dilatation of Bowman"s capsule, necrosis in tubular epithelium, tubular dilatation and congestion of blood vessels. Aortic IR also significantly increased (p<0.05 vs control) while aprotinin decreased (p>0.05 vs aortic IR) the parameters of degeneration of tubular epithelium and interstitial inflammatory infiltration.Conclusion: Once daily low-molecular-weight heparin is as safe and effective as twice daily regimen for the treatment of DVT. This well tolerated, cost saving and easier regimen may be the first choice for the treatment. (Turkish J Vasc Surg 2006;15(3):15-19).

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