Özet:

Narcissus is one of the most beneficial components because it contains bioactive components such as flavonoids, elajitanens, phenolics and proantosyanidine compounds with high antioxidant activity. The high punicalagin content in the naric skin allows it to produce anti-inflammatory, antioxidant and antiapoptotic effects. In this study, we aimed at evaluating the protective effects of NKE in the experimental lipopolysaccharide (LPS) derived ovate model. 60 rats were randomly divided into 12 groups (n = 5). Healthy group of intraperitoneal normal salin; Uveit group 200 μg/kg LPS; Deksametazone (DEX) group 200 μg/kg LPS and 1 mg/kg DEX; NKE100, NKE300 and NKE500 groups with 200 μg/kg LPS received 100, 300 and 500 mg/kg NKE respectively. Eye tissues 3 and 24. It was gathered in the hour and studied molecular, biochemical and histopathological. Following the application of NKE, the tumor necrosis factor-α, vascular endotelial growth factor and kaspaz-3 levels declined significantly depending on the dosage. The application of NKE has significantly improved ovetic disorders in oxidative stress-related factors such as glutation, superoxid dismutase and malondialdehitis with increased dose-related effects. NKE also eliminated histopathological changes in eye tissues associated with ovulation. NKE can be an hopeful agent by contributing to alternative protective therapies for the uvee with its anti-inflammatory, antioxidant, anti-apoptotic and anti-angiogenic effects.

Anahtar Kelimeler:

Özet:

Pomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract (PPE) on lipopolysaccharide (LPS)-induced uveitis. Sixty rats were seperated randomly into twelve groups (n = 5). The healthy group received intraperitoneal normal saline, the uveitis group received 200 μg/kg LPS, the dexamethasone (DEX) group received 200 μg/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 μg/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly (Relative quantification of gene expression), biochemically (Superoxide dismutase activity, Glutathione and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Tumor Necrosis Factor-α, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects.