Özet:

This study aims to investigate the impact of the swimming time, oxidative stress and oxidative stress related to the levels of proteins HO-1 and Nrf2 on the rates swimming until the extinction of selenium, which is an important antioxidant. For this purpose, 18 Wistar-Albino races male rats are divided into 2 groups, which will be 9 in each group. Before the experimental application began, the groups were swim for half an hour to get the rats used to swim. In the control group plasebo (serum physiological) and in the selenium group 0.8 mg/kg/day selenium was administered intraperitoneally for 20 days. At the end of the 20th day, the groups were swimming and recorded swimming times. The rats were sacrificed under anesthesia immediately after the swimming process. From blood, muscle and liver tissue samples, malondialdehitis (MDA), glutation (GSH) levels, catalase and glutation peroxidase (GSH-Px) enzyme activities have been determined. In muscle and liver tissue samples, the HO-1 and Nrf2 protein expression levels have been looked at using Western blot technique. According to the data obtained; there was no difference in the direction of swimming time between the groups (P>0.05). The level of MDA in the liver and muscle tissue has been shown to be significantly lower in the selenium group compared to the control group (P<0.05). A significant increase in the level of hepatic GSH in the selenium group has been observed according to the control group (P<0.05). A significant increase in muscle, plasma and liver tissue has been observed in the selenium group at the GSH-Px level according to the control group (P<0.05). Hepatic tissue HO-1 and Nrf2 protein expression levels were found significantly high in the selenium group (P<0.01). As a result, the application of selenium to swimming rates until its extinction has reduced oxidative stress and increased antioxidant activity and the levels of Nof2 and HO-1 protein expression, and has not affected the swimming times.

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