Abstract:

Objective: Breast cancer is one of the most frequently diagnosed cancer types in women in the world and in our country. It is known that it is the second leading cause of death in women worldwide. The aim of this study is to determine the antiproliferative, apoptotic and antioxidant effects of Melissa officinalis extract on breast cancer cell line (MCF-7). Methods: In the study, different concentrations of Melissa officinalis extract (1 µg/mL, 10 µg/mL, 100 µg/mL and 1000 µg/mL) were applied to the MCF-7 cell line. Cytotoxic activities were determined using the MTT method at 24 hours, and apoptotic activities were determined using the muse annexin V method. To determine the biochemical parameters, 1 µg/mL, 10 µg/mL, 100 µg/mL and 1000 µg/mL doses were applied to the cells and then cell lysates were obtained. Firstly, the protein level of the cell was calculated with the prepared samples. Then, TOS, TAS, OSI, SOD, GPx activity determination, MDA and NO analyzes were investigated. Results: When the results were evaluated, it was determined in our study that Melissa officinalis extract decreased MCF-7 cell proliferation depending on dose and time. At the same time, the increase in apoptosis we obtained explains this proliferation inhibition. The antioxidant results that emerged within the framework of the research also support the inhibition of proliferation and also the increase of apoptosis. Conclusion: When all the statistical data are interpreted, it has been determined by the current research that the molecular anticarcinogenic mechanisms of Melissa officinalis extract in MCF-7 cells will contribute to the development of new chemotherapeutic and chemopreventive agents for breast cancer treatment by working alone or in combination with chemotherapeutic agents.

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