Abstract:

Objective: In the present study, the levels of antioxidant systems such as glucose-6-phosphate dehydrogenase (G6PDH), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and, also reduced glutathione (GSH) in liver and breast tissues were examined in order to obtain basal data for subsequent toxicological investigations. Additionally, the level of thiobarbituric acid reactive substance (TBARS) was measured in liver and breast tissues as an index of lipid peroxidation. Materials and Methods: Sixty-six apparently healthy mice (36 male, 30 female) had normal liver and breast tissues (histopathological data) were taken into the study. The tissues were homogenized with ice-cold 1.15% KCI. The activities of antioxidant enzymes, the levels of GSH and TBARS were measured as spectrophotometric in the supernatant obtained from centrifugation at 14,000 rpm. Results: The levels of antioxidant systems and TBARS in the liver were significantly higher than those found in breast tissue of female and male mice (P < 0.05). However, the levels of CAT, SOD, GST, GSH and TBARS in breast tissue except G6PDH enzyme were not significantly different between female and male mice (P > 0.05). On the other hand, the levels of CAT, SOD, GST, GSH and TBARS in male liver except G6PDH enzyme were higher than female liver (P < 0.05). Conclusion: Results suggest that the antioxidant defense of the liver tissue was higher compared to breast tissue possibly due to a compensatory response to various toxic substances in the liver and thereby protects the cells against oxidative damage.

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