Abstract:

Purpose: In the study, the aim was to study the relationship of the protective effect of alpha lipoic acid (ALA) on changes in the kidney tissues of adriamisin (ADR) applied to dynamine-dependent protein 1 (DRP1). In the study, 28 male rats of the Sprague-Dawley gender were used. The animals were divided into four equal groups. No application was made to the control group for the 28 days of the test period. The ADR group was given a single dose of 15 mg/kg of ADR intraperitoneal (i.p). ADR+ALA group was given a single dose of ADR 15 mg/kg per day after 50 mg/kg/day dose of ALA oral overdose. In the ALA group, 50 mg/kg/day dose of ALA was administered orally overnight. At the end of the test, the rats were decaptivated. After decapitation, kidney tissues were quickly removed and used for histological and quantitative RT-PCR analyses. Results: In the kidney cuts of the ADR group, inflammatory cells have increased, structural changes have been observed, such as the increase in the bowman empty, the accumulation of hyalin in the tubular lumen. With the application of ALA, a decrease in the vast majority of histopathological findings was observed. In the ADR group, DRP1 immune reactiveness increased significantly compared to the control group, while in the ADR+ALA group, ADR group increased significantly. A statistically significant increase in the DRP1, BAX and CASP3 mRNA levels in the ADR group compared to the control group was observed. In the ADR+ALA group, a significant decrease in the DRP1, BAX and CASP3 mRNA levels compared to the ADR group was observed. This study found that ADR, along with structural damage in kidney tissues, leads to an increase in apoptosis and DRP1 expression, and that ALA, regulating apoptosis and DRP1 activities, provides a substantial protection against these harmful effects.

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