Abstract:

Nefrotoxicity is the most common side effect of sisplatin, a large-scale anti-ineoplastic drug. Therefore, in patients with recurrence in renal functions after taking a dose of cysplatin, the use of cysplatin is limited by 25-35%. Nefrotoxicity caused by the sickness is associated with increased oxidative stress in the kidneys. In this study, we studied how melatonin, as an antioxidant in the cyplatin-generated nefrotoxicity, works against cyplatin-generated nefrotoxicity at biochemical, histopathological and molecular levels. This study, a single dose of intraperitonal (i.p.) A rat model of nefrotoxicity created by the application of siplatin shows that the application of melatonin has a protective effect on the kidneys. The serum urine and creatine values in melatonin given animals have shown a significant decrease in renal dysfunction compared to the group injected by sisplatin. The levels of MDA and 8-iso-PGF2α in the kidney tissue were significantly high compared to the control group in the group of siplatin. In rats treated with melatonin, the level of SIRT-1 protein expression is markedly regulated in the direction of increase, while the level of expression of NAMPT, COX-2, TGF-β1 and TNF-α protein has decreased. Histopathological findings have shown that pre-treatment with melatonin also prevents the majority of changes generated by cysplatin, mainly by tubular necrosis derived from cysplatin. The results of this study show that melatonin in kidney damage created with cisplatin can provide healing by reducing the burning through the COX-2, TGF-β1 and TNF-α inhibition by regulating the NAMPT/SIRT-1 signal path.