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Aim: investigate and characterize the presence of polypeptide pool in blood plasma and tumor tissues in patients with bladder cancer at different stages of the disease. Materials and Methods: Patients with bladder cancer at different stages participated in the trial. The diagnosis was based on X-ray, endoscopic, clinical research methods with morphological verification. None of the patients treated the oncological diseases for a preliminary study. Plasma blood was taken in the preoperative period. Cancer and healthy tissues of the bladder were selected for biopsy, immediately after surgery. Isolation of polypeptide pool in blood plasma and tissue homogenates was carried out using the Nikolaichuk method. The qualitative composition of the protein component of polypeptide pool fraction was investigated using chromatography, using a column with Sephadex G 15. Results: In the course of the study, reliable changes in the content of medium molecules in blood plasma and tumor homogenates in patients with bladder cancer have been shown. The changes correlate with the stage of the disease. A qualitative analysis of the composition of the protein component of medium mass molecules was carried out, using chromatography. Conclusions:The fraction of the medium mass molecules from the blood plasma and tumor tissues of patients with various stages of bladder cancer was obtained. The quantitative and qualitative content of the fraction of polypeptide pool in blood plasma and tumor homogenates has been investigated. The content of polypeptide pool in the blood plasma and tumor tissues of patients with bladder cancer is significantly increased compared with the blood plasma of healthy men and compared to healthy tissues of the bladder. The qualitative composition of the polypeptide pool in blood plasma and tumor homogenates differs from that in the plasma of healthy patients or in healthy tissues of the bladder   Author Biographies Viktor Dmytryk, ESC "Institute of Biology and Medicine" Taras Shevchenko National University of Kyiv Akademika Hlushkova ave., 2, Kyiv, Ukraine, 03127 Postgraduate student Department of Biochemistry

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