Özet:

Purpose: Candida parapsilosis type complex and Lodderomyces elongisporus isolates may have differences in terms of virulence, prevalence and antifungal sensitivity profiles. These species are difficult to identify by biochemical methods. Therefore, more efficient methods are needed in terms of applicability, time and cost. The purpose of this study is to evaluate the diagnostic performance of the MALDI-TOF MS method in the distinction of the isolates of the C. parapsilosis type complex and L. elongisporus. Method: In the presented study, a total of 32 reference insulates containing C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6) and L. elongisporus (n=11) were identified by the MALDI-TOF MS method. Results: Candida parapsylosis type complex and L. The type name of the 31 (93.7%) isolate belonging to elongisporus was correctly defined. The name of the total 24 (%75) types of isolates containing eight (%100) C. parapsilosis, 5 (%83) C. metapsilosis, 5 (%71) C. orthopsilosis and 6 (%54) L. elongisporus is identified by score values ranging from 1.7-2.14. When referring to the ≥1.7 score for secure identification, the sensitivity and specificity of the MALDI-TOF MS method was determined at 54.5-100% and 96.3-100% respectively. The result: Candida parapsylosis type complex and L. In contrast to the demonstration that MALDI-TOF MS is an effective method in the fast molecular phenotypic diagnosis of species with differentiation by biochemical methods such as elongisporus, it is clear that the method is optimized to identify the species at a safer score range and requires a broader MS library.

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