Abstract:

Chakok ardu (Pyrus elaeagnifila Pallas) is a wild ardue that grows naturally. After the harvest, it can grow up and be defeated. This study covers the determination of the total phenolic substance, total flavanoid substance, total ascorbic acid, glutation (GSH) and superoxid dismutase (SOD); antioxidant activity, antimicrobial activity and mutagenic activity. According to the obtained data, the total phenolic content of the samples is 174.2 mg GAE (galic acid equivalent)/100g extract, the total flavanoid content is 44.9 mg RE (rutin equivalent)/100g extract, the total ascorbic acid content is 14.5 mg AE (ascorbic acid equivalent)/100g extract, the iron ion reducing antioxidant power (FRAP) value is 515. 6 μmol Fe (II)/g extract, 2.2'-Azino-bis 3-ethyl benzotiazolin-6-sulfonic acid (ABTS) spray activity 53.5%, 1,1-difenyl-2-pikrilhydrazil (DPPH) spray activity 48.2%, GSH content 3.98 μM and SOD content 6.88 U/ml have been detected. The fruit extract has been determined to have an antimicrobial activity in different proportions against the test bacteria. It has been tested whether six different concentrations (10, 20, 40, 60, 80 and 100 μL/plate) of fruit extract, determined in the preliminary trials, have demonstrated mutogenic activity against Salmonella typhimurium TA 98 and TA 100. No statistically significant mutogenic effect was observed over TA 98 and TA 100 shakes in any of the trial doses. This study has shown that the shake shake has important bioactive components, antioxidant and antimicrobial activity.

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