Özet:

Objective: This study aimed to determine prevalence, antimicrobial susceptibility profile and the genetic basis to antimicrobial resistance, targeting blaTEM gene expression of diarrheagenic Escherichia coli among patients suffering from gastroenteritis in Kitale County Referral Hospital. Methods: A cross-sectional study design was adopted. A total of 103 fecal specimens were collected from participants ranging in age from two weeks to 82 years. E. coli was isolated and identified based on phenotypic and biochemical properties. Antimicrobial susceptibility was determined by Kirby-Bauer disk diffusion method. Polymerase chain reaction was used to detect the presence of blaTEM gene. Results: The prevalence of E. coli was 90.2% and age of the patient explained 53% of variation in prevalence. Isolates of diarrheagenic E. coli showed varied degree of susceptibility with sulfamethoxazole at 97%, co-trimoxazole 96%, ampicillin 84%, chloramphenicol 27%, tetracycline 16%, kanamycin 10% and streptomycin 9%. However, E. coli was highly sensitive to gentamicin at 96.8%. Approximately 42.2% of E. coli isolates were multidrug resistant to sulfamethoxazole, co-trimoxazole, ampicillin, chloramphenicol, tetracycline, kanamycin and streptomycin. All isolates that were resistant to ampicillin harbored blaTEM gene suggesting genetic mediation. Conclusion: The observed pattern of resistance to antibiotics points to the need to regulate their use and arrest buildup of resistant genes within the population. J Microbiol Infect Dis 2016;6(3): 107-112  Antimicrobial resistance, antimicrobial susceptibility, blaTEM gene, Escherichia coli

Anahtar Kelimeler: