Özet:

In the study, methotrexate (MTX) is intended to investigate the effect of the engineering application on oxidative stress and antioxidant enzyme activities in the blood tissue of the wheat applied. Wistar-Albino male wheels are divided into four groups in the form of control group, engineer applied group, MTX applied group and MTX+ engineer applied group. MTX and engineering applications are launched simultaneously. The engineering 1.5 g/kg/day body weight dosage was administered through the gage for 9 days, while MTX was administered intraperitoneally for 7 mg/kg/day body weight dosage for 3 days. At the end of the nine-day trial period, all the rats were sacrificed and blood tissue samples were taken to tubes containing anticoagulants. In the study, in order to determine the engineer’s effects on MTX-related damage, malondialdehyd (MDA), reduced glutation (GSH) levels, catalase (KAT), glutation peroxidase (GSH-Px) and superoxid dismutaz (SOD) activities were measured. In comparison with the control group on MTX, MDA (P<0.001) levels were significantly increased, while GSH (P<0.001) levels and KAT (P=0.001), GSH-Px (P=0.001) and SOD (P<0.001) activities were significantly decreased according to the control group. In the MTX+Enginar applied group, the values of all parameters approached the values of the control group. The MTX+enginar applied group compared to the MTX applied group, the MDA levels fell, the GSH levels increased with the KAT and GSH-Px activities, and the SOD activities did not change. Finally, the engineer has found that it can be a natural antioxidant that can be used to reduce the toxicity caused by MTX.

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