Abstract:

Purpose: Doksorubin is an anthracycline and has been shown to have similar nefrotoxic effects in various animal models and human studies. In this study, docsorubisin was aimed at studying the effects of benfotiam on changes in the kidney tissues of the rats applied. In the study, 24 Wistar albino male rats were used. The animals were divided into four equal groups. No application was made to the control group for 14 days of the test period. A single dose of 10 mg/kg intraperitoneal (i.p) was administered to the Doksorubisin group. Doksorubin + Benfotiamine group was given orally at a dose of 70 mg/kg/day after a single dose of 10 mg/kg of doxorubin. The benfotiamine group was administered orally at a dose of 70 mg/kg/day. At the end of the test, the mice was decaptivated and the kidney tissues were removed. With the routine tracking process, the tissues were buried in paraffin blocks. On cuts taken from blocks, the avidin-biotin-peroxidase method was applied for Bax and kaspaz-3 immune reaction. The level of malondialdehitis (MDA) was determined spectrophotometrically. Results: Bax and kaspaz-3 immune reactivity was observed in control and +1 prevalence in benfotiamine groups. In comparison with control groups, an increase in Bax and Kaspaz-3 immune reactiveness in the Doksorubisin group was observed and the prevalence was assessed at +3. Compared to the Doksorubisin group, a decrease in Bax and Kaspaz-3 immune reactiveness in the Doksorubisin + Benfotiamin group was observed and evaluated at +2 prevalence. The level of MDA was found significantly increased in the kidney tissues when compared to the group given to Doksorubisin, the control group and benfotiamine. Compared to the Doksorubisin group, the MDA levels in the given group of Doksorubisin + benfotiamine were found significantly decreased. The result: This study has shown that docsorubis induces apoptosis in kidney tissues and that benfotiam has anti-apoptotic and antioxidant effects against the effects of docsorubis.

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