Özet:

Purpose: The purpose of this study is to create reference intervals (RIs) according to laboratory data in a population and to evaluate the status of vitamin B12 (B12) and folate in relation to RI for all age and gender groups. Method: Laboratory data was obtained retrospectively from the Laboratory Information Management System of the Fisheries State Hospital. 36,284 patients between the ages of eighteen and 80 were selected (70% for women and 29,8% for men). The data was divided into six sub-age groups (18-30, 31-40, 41-50, 51-60, 61-70 and 71-80 years old). B12 and folate concentrations were measured by the ARCHITECT i2000sr (Abbott Diagnostics, Abbott Park, IL, USA) autoanalyst. The end values were dropped using IBM SPSS. RIs was calculated using non-parametric indirect method in 95% distribution. Results: Results of 20,850 patients for B12 and 14,183 patients for folate were evaluated. The average age of B12 and folate patients was 48,9±16,3 and 49,7±16,6 respectively. The average ± standard deviation concentrations of B12 and folate were 220±77 pmol/L and 13,9±6,5 nmol/L respectively. 95% RIs for the entire population, 97-397 pmol/L for vitamin B12 and 5,17-30,9 pmol/L for folic acid were calculated. There was a statistically significant difference between women and men for B12 and folate. There was a significant difference between the age groups of RIs for folate. However, there was no significant difference in B12 concentrations. Result: This study found a difference between the RIs recommended by the manufacturer and the RIs of our own population. Our findings show that it is important for each laboratory to determine its own reference range.

Anahtar Kelimeler: