Özet:

The aim of this study is to determine the etiological spectrum of pathogens found in the nasal cavity using MALDI-TOF MS in pneumonic cows. The study material consisted of 64 cows between 2 and 7 years of age, 39 cows between 30 and 85 days of age and 20 healthy cows and cows from 24 separate farms from the different districts of İzmir. After the clinical trials of all animals were carried out, the nose sample was taken using coal amies transported nutritious swap. The clinical examination was also statistically high (<0.001) in cases with respiratory frequency, heart rate and rectal temperature indicating symptoms of pneumonia. With MALDI-TOF MS, 17 different bacteria were isolated from cattle samples. Accordingly; Escherichia coli (E. coli) (32.8%) and Staphylococcus lentus (S. Lentus (% 28.1), Staphylococcus sciuri (S. sciuri) (% 21.8) were the most isolated pathogens. Thirty different bacteria were isolated. Accordingly, E. coli (46.1%), S. lentus (41%), Staphylococcus xylosus (S. xylosus) and S. sciuri (7.6%) were the most isolated bacteria. For mycoplasma isolation and molecular characterization, PCR was made. In 29 of the samples taken from cattle and pigs, Mycoplasma spp. Mycobacterium bovis (M. bovis) was isolated. Most of the factors that mix the infection with M. bovis were S. sciuri (% 12.5) and E. coli (% 12.5). While these findings reveal the spectrum of pneumonia factors in our area, MALDI-TOF MS shows that pathogens in the nose empty can be detected quickly and reliably.

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