Özet:

Streptococcus parauberis is an alpha-hemolytic gram positive coccoid bacterium belonging to the Streptococcaceae family. This bacterium cause streptococcosis is a major disease in cultured fish due to the intensification of aquaculture and causes significant economic losses in fish farm industry. In our study, we isolated a total of 37 lactic acid bacteria from wild fish (Sander lucioperca, Carassius gibelio, Cyprinus carpio) of Lake Eğirdir and cultured fish (Oncorhynchus mykiss, Walbaum) of farm in Turkey. For the isolation of bacteria phenotypic and biochemical characteristics of the 37 isolates obtained from the colonies grown on MRS, TSA and M17 agar characterized by determining colony morphology, cell morphology, motility, gram staining and the production of cytochrome oxidase and catalase. Further biochemical characteristics were determined using conventional tests according to Bergey’s Manual of Systematic Bacteriology. From the isolated lactic acid bacteria 25 of were identified as S. parauberis, 2 of as Vagococcus sp., 2 of as Lactococcus garvieae and 5 of as Lactococcus lactis by culture-based, biochemical test and 16Sr RNA gene sequencing techniques but 3 of them couldn’t (F65, F49 and F50) identified.  In this study, the main objective of us is to identify S. parauberis with conventional and 16Sr RNA gene sequencing techniques from wild fish in Lake Eğirdir and in cultured fish in fish farm of Egirdir-Turkey. 

Anahtar Kelimeler: