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Bu çalışma, deneysel olarak kronik florozis oluşturulan ratlarda vitamin C (vit C) ve E (vit E)'nin serum protein fraksiyonları üzerindeki etkilerini araştırmak amacıyla planlandı. Materyal olarak Wistar-Albino ratlar kullanıldı (Her biri 8 rat içeren, 8 grup). Deneme grupları kontrol grubu, deneysel florozis grubu, koruma grupları (16 hafta boyunca, içme suyu içeren günlük 150 ppm NaF içeren içme suyu/ad-libitum ile birlikte vit C (100 mg / kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) ve tedavi grupları (16 hafta boyunca 150 ppm NaF içeren içme suyu/ad-libitum verildikten sonra tedavi amacıyla 4 hafta vit C (100 mg/kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) olarak oluşturuldu. Deneme sonunda kan örnekleri toplandı ve serumlar alındı. Bu örneklerde serum protein fraksiyonları selüloz-asetat elektroforezi ile belirlendi. Deneysel florozis grubunda total protein yüzde gram açısından, alfa-1’in önemli oranda azaldığı ve beta globülinlerin arttığı tespit edildi. Korunma ve tedavi amacıyla vitamin C, vitamin E ve vitamin C+vitamin E birlikte verildiği gruplarda, değişen bu değerlerin kontrol grubuna yaklaştığı görüldü.
This study was experimentally planned to investigate the effects of vitamin C (vitamin C) and E (vitamin E) on serum protein fractions in rates that are created with chronic florosis. Wistar-Albino wheels were used as material (every containing 8 wheels, 8 groups). Test groups; control group, experimental florosis group, protective groups (16 weeks, drinking water containing 150 ppm of NaF/ad-libitum, vit C (100 mg/kg), vit E (300 mg/kg) and vit C + vit E (100 mg/kg + 300 mg/kg)) and therapeutic groups (16 weeks, drinking water containing 150 ppm of NaF/ad-libitum, for the purposes of treatment, were formed as 4 weeks of vit C (100 mg/kg), vit E (300 mg/kg) and vit C + vit E (100 mg/kg + 300 mg/kg). At the end of the test, blood samples were collected and serums were taken. In these examples, serum protein fractions were determined by cellulose-acetate electrophoresis. In the experimental florosis group, the total protein percentage of gram was found that alpha-1 was significantly reduced and beta globulin increased. In groups where vitamin C, vitamin E and vitamin C + vitamin E were given together for protection and treatment, these variable values were found to be closer to the control group.
The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.
Field : Sağlık Bilimleri
Journal Type : Ulusal
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