Özet:

Background: Ceratonia siliqua L., a member of the family Leguminosae, has been reported to have antimicrobial, antifungal and cytotoxic activity (carob, kharnub). In this study, the antiproliferative and cytotoxic effects of extracts prepared with methanol and ethanol from the carob leaves and fruits on HeLa cells were investigated. Additionally, 3T3, embryonic mouse fibroblast cell line, was used as a control. Material and Methods: For these purposes, after treatment of the extracts at different concentrations (200-800μg/mL) for 48 hours, viable cell counts (proliferation, MTT method), mitotic index (mitotic activity) and labeling index (DNA synthesis) were determined. In addition, the phenolic component compositions in the extracts were also determined by LC-MS / MS. Results: According to the findings, the methanolic and ethanolic leaf extracts of carob bean suppressed mitotic activity and proliferation by inhibiting the DNA synthesis of human cervical cancer HeLa cells. However, these extracts also exhibited a cytotoxic effect against normal fibroblast 3T3 cells. Ethanolic fruit extract reduced the DNA synthesis of HeLa cells. Conclusions: The active substances or preparations obtaining from the C. siliqua plant have the possibility of being an anticancer agent, whereas their cytotoxic effects on the normal cells should not be overlooked.

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