Özet:

Micropropagation of garden crops (steppe cherry "Pamjati Lewandovskogo", remontant raspberry "Hercules", chrysanthemum korean (multiflora group) "Harmoniya" and "Daphna") was performed on Murashige and Skoog (MS) medium supplemented with different auxin and cytokinin growth regulators. Agar and potato starch at different concentrations were used as a medium amendment. The combination of 4.0 gr/l agar and 20.0 gr/l starch has been found to be very effective. The addition of antibiotic (amoxicillin) to the culture medium at the micropropagation stage prevented contamination of regenerants and improved their growth. Keywords: Cherry; raspberry; chrysanthemum Korean; tissue culture; potato starch; antibiotic; multiplication References: Ayub, R. A., & Reis, L. (2016). Organogenesis inhibition of strawberry, cultivar ?Festival?, by the kanamycin antibiotic. Acta Horticulturae, 1113, 147-150. Doi: 10.17660/ActaHortic.2016.1113.21. Cornu, D., & Michel, M. F. (1987). Bacteria contaminants in shoot cultures of Prunus avium L. choice and phytotoxicity of antibiotics. Acta Horticulturae, 212, 83-86. Doi: 10.17660/ActaHortic.1987.212.10. Georgieva, L., Tsvetkov, I., Georgieva M., & Kondakova, V. (2016). New protocol for in vitro propagation of berry plants by tis bioreactor. Bulgarian Journal of Agricultural Science, 22 (5), 745-751. Jerzy, M., Zalewska, M., & Tymoszuk, A. (2015). Effect of kinetin on the elongation of adventitious shoots regenerated in vitro from ligulate florets in chrysanthemum × grandiflorum Ramat. Kitam. Acta Horticulturae,1083, 577-584. Doi: 10.17660/ActaHortic.2015.1083.77. Metodicheskie rekomendacii po ispol'zovaniyu biotekhnologicheskih metodov v rabote s plodovymi, yagodnymi i dekorativnymi kul'turami. (2005). Orel (in Russian). Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. Doi: 10.1111/j.1399-3054.1962.tb08052.x Paprstein, F., & Sedlak, J. (2017). Identification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol. Acta Horticulturae, 1155, 161-164. Doi: 10.17660/ActaHortic.2017.1155.22. Reed, B. M., Mentzer, J., Tanprasert, P., & Yu, X. (1998). Internal bacterial contamination of micropropogated hazelnut: identification and antibiotic treatment. In: Cassells A. C. (eds) Patogen and microbial contamination management in micropropagation. Developmenrs in Plant Pathology, 12: 169-174. Doi: 10.1007/978-94-015-8951-2_20. Rerez-Jimenez, M., Carrillo-Navarro, A., & Cos-Terrer, J. (2012). Regeneration of peach (Prunus persica L. Batsch) cultivars and Prunus persica × Prunus dulcis rootstocks via organogenesis. Plant Cell Tissue and Organ Culture, 108(1), 55-62. Doi: 10.1007/s11240-011-0011-y. Sedlak, J., & Paprstein, F. (2008). In vitro shoot proliferation of sweet cherry cultivars Karesova and Rivan. Horticultural Science, 35(3), 95-98. Doi: 10.17221/6/2008-HORTSCI. Sriskandarajah, S., & Lundquist, P.-O. (2009). High frequency shoot organogenesis and somatic embryogenesis in juvenile and adult tissues of seabuckhorn (Hippophae rhamnoides L.). Plant Cell, Tissue and Organ Culture, 99, 259-268. Doi: 10.1007/s11240-009-9597-8.

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