Abstract:

Background: Cancer is one of the diseases that developed by uncontrolled proliferation of cells and become more common every year. The inadequacy of existing treatment methods, the need for new methods with lesser side effects have increased. Phytotherapy, which has become widespread as one of the cancer treatment methods in recent years, has created the most remarkable candidate and a rapidly developing field. In this study, based on the phytotherapy method, the anticancer activity of the green outer bark of the pican nut (Carya illinoinensis) was investigated. Materials and Methods: The anticancer activity of pican nut green bark on various cancer cell lines was tested using the cell culture method. In this context, the cytotoxic value of the green-shell hexane extract of pecan nut was tested on PC-3, DU-145, PNT1-A, HT-29, HCT-116 and HUVEC cell lines. The percentage of apoptotic cells was investigated with Annexin V/PI staining and cell cycle with PI staining. Results: According to the experimental results, it was determined that pican nut green-outer shell hexa-ne extract showed highest cytotoxic effect on PC-3, which is the prostate cancer cell line (IC50: 40.32µg/ml). The percentage of apoptotic cells was found to be 94% and the of cells retained in the G0/G1 phase was 59.2%. Conclusions: As a result of this study, it was determined that PC-3 cells suppressed cell division and triggered apoptosis in cells. Based on these results, it is predicted that the green-outer shell of pican nut is an alternative plant candidate that can be used in the treatment of cancer in the pharmacological field and will guide the studies to be done in this field. Keywords: Pecan Nut, Carya illinoinensis, Anticancer, Apoptosis, Cell Cycle