In this study, the production of single cell protein from waste of Citrullus vulgaris Schrad. (Watermelon) and the optimization of production were investigated. Single Cell Protein (SCP) was produced by inocolation of activated yeast cells (Candida utilis and Saccharomyces cerevisiae) into watermelon juice. In nutrient medium with watermelon juice, the effect of different concentration, temperature, glucose, fructose, sucrose and pH was investigated, and the most appropriate SCP production terms were investigated. As a result of research: it is determined that S. cerevisiae exhibited the best growing in the medium including 30% of watermelon juice and C. utilis exhibited the best growing in the medium including 20% of watermelon juice. For C. utilis, the most appropriate temperature was 30 ºC; for S. cerevisiae, the similar results were obtained at the temperatures (25, 30 and 35 ºC) applied in the study. In the study showed what the effect of carbohydrates to SCP production was, it was determined that S. cerevisiae 1 %, C. utilis 2 % were appropriate in the medium including glucose; S. cerevisiae 2 %, C. utilis 3 % in the medium including fructose; and S. cerevisiae 3 %, C. utilis 2 % in the medium including sucrose. It was found that pH 6 was the most appropriate pH for S. cerevisiae and C. utilis and this pH was appropriate for SCP production
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