Bu çalışmada yangısal süreçte önemli görevleri olan adenozin deaminazın, subklinik mastitisli ineklerde serum ve süt adenozin ademinaz (ADA) düzeylerinin belirlenmesi ve bu enzimin somatik hücre sayısı ile ilişkisinin ortaya konması amaçlandı. Çalışma grubunu oluşturan Holstein inekler subklinik mastitisli (n=40) ve sağlıklı inekler (n=15) olarak iki gruba ayrıldı. Kaliforniya mastitis testi (CMT) sonucunda şüpheli sonuç veren ve herhangi bir klinik mastitis bulgusu bulunmayan inekler subklinik mastitis grubunu, CMT negatif olan inekler ise kontrol grubunu oluşturdu. Süt örneklerindeki somatik hücre sayıları flow sitometri ile belirlendi. Subklinik mastitis grubunda serum ve süt ADA değerleri sırasıyla 8,11 ve 3,38 IU/L ölçüldü. Somatik hücre sayıları subklinik mastitis ve kontrol grubunda sırasıyla 248x103 ve 132x103 bulundu (p<0.01). Süt ADA ile somatik hücre sayısı arasında da pozitif korelasyon bulundu (p<0.01, r=0.611). Sonuç olarak, adenozin deaminazın ineklerde subklinik mastitis olgularının tanısında bir belirteç olarak kullanılabileceği kanısına varıldı.
This study was aimed at determining the levels of serum and milk adenozine adenozine (ADA) levels of adenozine deaminase, which plays an important role in the fire process, and the association of this enzyme with the somatic number of cells. The Holstein cow, which formed the study group, was divided into two groups: subclinical mastitis (n=40) and healthy cow (n=15). The California Mastitis Test (CMT) resulted in a suspicious result and the cows that did not find any clinical mastitis found formed the subclinical mastitis group, while the cows with CMT negative formed the control group. The somatic number of cells in milk samples was determined by flow sitometry. In the subclinical mastitis group, the serum and milk ADA values were measured respectively 8.11 and 3.38 IU/L. The number of somatic cells was found in the subclinical mastitis and control group, respectively, 248x103 and 132x103 (p<0.01). There was also a positive correlation between the milk ADA and the somatic cell number (p<0.01, r=0.611). As a result, adenosine was found to be a indicator that deaminase can be used in the diagnosis of subclinical mastitis in cows.
In this study, we aimed to determine the levels of adenosine deaminase (ADA), which plays important role in inflammatory process in sera and milk in cows with subclinical mastitis and state the relationship between this enzyme with somatic cell count. Holstein cows which was consisted the study group allocated into two groups subclinical mastitis (n = 40) and healthy cows (n = 15). After performing the California Mastitis Test (CMT), the cows resulted suspicious; included the study group, the cows which showed CMT as negative constitute the control group. Somatic cell counts were determined with flow cytometry assay. In subclinical mastitis group, serum and milk ADA values were 8.11 and 3.38 IU / L, respectively. Somatic cell counts were 248x103 and 132x103 in subclinical mastitis and control groups, respectively (p <0.01). There was a positive correlation between milk ADA and somatic cell count (p <0.01, r = 0.611). As a result, adenosine deaminase can be used as a marker in the diagnosis of subclinical mastitis in cows.
Alan : Sağlık Bilimleri
Dergi Türü : other
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