Görüntüleme
13
İndirme
4
Bu çalışmada, ratlarda sisplatin (SP) kaynaklı hepatotoksisite üzerine zingeron’ un (ZO) etkilerinin araştırılması amaçlandı. Çalışmada 35 adet Sprague Dawley erkek rat kullanıldı. Hayvanlar, her grupta 7 rat olacak şekilde 5 gruba ayrıldı. 1.Grup (Kontrol): Sadece oral serum fizyolojik (SF, 7 gün) verildi. 2. Grup (SP): Tek doz 7 mg/kg SP periton içi (i.p.) uygulandı. 3.Grup (ZO): ZO 7 gün boyunca 50 mg/kg/gün dozunda oral verildi. 4. Grup (SP+ZO 25): Tek doz SP uygulamasından 30 dk sonra 25 mg/kg/gün dozunda ZO verilmeye başlandı ve 7 gün devam etti. 5.Grup (SP+ZO 50): Tek doz SP uygulamasından 30 dk sonra 50 mg/kg/gün dozunda ZO verildi ve 7 gün devam etti. Alanin transaminaz (ALT), aspartat transaminaz (AST) ve alkalen fosfataz (ALP) aktivitelerinin SP uygulaması ile arttığı, antioksidan enzimlerden süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (KAT) aktiviteleri ile non-enzimatik antioksidan olan redükte glutatyon (GSH) seviyelerinin SP grubunda düştüğü, malondialdehit (MDA) düzeyinin arttığı görülmüş, ZO’ un ise antioksidan sistemi desteklediği ve MDA düzeylerini azalttığı gözlenmiştir. SP uygulamasının arginaz aktivitesini düşürürken nitrik oksit (NO), 8-hidroksi-2'-deoksiguanozin (8-OHdG), sistein aspartat spesifik proteaz-3 (kaspaz-3), tümör nekroz faktör-alfa (TNF-α), nükleer faktör kappa B (NF-ĸB) ve B-hücreli lenfoma-3 (Bcl-3) seviyelerini artırdığı, ZO’ un bu parametrelerde iyileşme sağladığı saptandı.
In this study, it was aimed at investigating the effects of zingeron (ZO) on hepatotoxicity caused by siplatin (SP) in rats. In the study, 35 Sprague Dawley male rat were used. The animals were divided into 5 groups in order to be 7 rat in each group. 1.Group (Control): Only oral serum physiological (SF, 7 days) was given. 2nd Group (SP): Single dose of 7 mg/kg SP peritoneal (i.p.) was implemented. 3.Group (ZO): ZO was administered orally in a dose of 50 mg/kg/day for 7 days. The fourth. Group (SP+ZO 25): 30 minutes after single dose of SP, 25 mg/kg/day dose of ZO began to be given and continued for 7 days. Group 5 (SP+ZO 50): A single dose of ZO was given in 50 mg/kg/day dose 30 minutes after the application of SP and continued for 7 days. Alanin transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) activity increased with the application of SP, the levels of reduced glutathion (GSH) in the SP group fell from antioxidant enzymes to superoxidant dismutase (SOD), glutathion peroxidase (GPx), catalase (KAT) activity and non-enzimatic antioxidant, the levels of malondaldehyde (MDA) increased, the ZO supported the antioxidant system and reduced the levels of MDA. The application of SP increased the levels of nitric oxide (NO), 8-hydroxy-2'-deoxiguanozin (8-OHdG), cystin aspartate specific protease-3 (kaspaz-3), tumor necrosis factor-alfa (TNF-α), nuclear factor cappa B (NF-ƒB) and B-celled lymphoma-3 (Bcl-3) while reducing the activity of arginase.
This study was aimed to investigate the protective effects of zingerone (ZO) on cisplatin (CP)-induced hepatotoxicity in rats. Thirty-five (35) Sprague Dawley male rats were used in the study; the animals were divided into 5 groups of 7 rats in each group. Group 1 (Control): received only oral serum physiologic (SP, for 7 days). Group 2 (CP): received a single dose of 7 mg/kg intraperitoneal (i.p.) of CP. Group 3 (ZO): Received daily oral doses of ZO at 50 mg/kg/day for 7 days. Group 4 (CP+ZO 25): Oral administration of ZO at 25 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Group 5 (CP+ZO 50): Oral administration of ZO at 50 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) activities increased due to CP administration. Also, enzymatic antioxidants; superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) level were shown to had decreased upon CP administration. After ZO was supported by antioxidant system and decreased malondialdehyde (MDA) levels. It was determined that CP administration increased nitric oxide (NO), 8-hydroxy-2'- deoxyguanosine (8-OHdG), cysteine aspartate specific protease-3 (caspase-3), tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB) and B-cell lymphoma-3 (Bcl-3) levels while arginase activity reduced, ZO administrations could have been the main cause of the improved effects on the parameters.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
Makale Detay 
Benzer Makaleler
PDF Görüntüle
Dergi Bilgisi
Eseri Dinleyin
Alıntı Yap
Bu Sayfayı Yazdırın
Paylaş
Mendeley
