Abstract:

Chemical, biological and physical factors may cause cytotoxicity by affecting cells at different degrees. To understand the biological characteristic of a compound, it is essential to determine its toxic or non-toxic effects. In vitro cytotoxicity assays (cell viability assays) are cell culture based measuring methods that usually used for either evaluating possible drug candidates or investigating cytotoxic profiles of some compounds. It is possible to assess many compounds in a short period of time and obtain fundamental information necessary for further animal experiments through this methods. There are many cell viability assay methods exist based on colorimetric, enzymatic, luminometric principals etc that have different mechanisms and varied sensitivity. The popular assays, such as MTT, MTS, XTT, WST, LDH enzyme assay, alamar blue assay, ATP bioluminescent assay have summarized in this rewiev. 

Keywords: