Özet:

Objective: Işkın (Rheum ribes L.), China, India, Iran and Turkey are grow in the wild and are used for many medical purposes. The aim of this study is to investigate the anti-cancer effectiveness of ethanol extracts of the roots, stems and bark of the Rheum ribes L against malignant melanoma cells. Material and Method: Root, stem and bark parts of the plant dried at room temperature were powdered with a mixer and incubated with 50% ethanol for 24 hours. In the extracts obtained after the organic phase is evaporated in a rotary evaporator and the water phase is separated in the lyophilizer; firstly, total phenol and total flavanoid content level and total antioxidant capacity were measured by photometric methods. Cultured malignant melanoma cells (B16F10) were incubated at different concentrations of these extracts for 24 hours. After incubation; cytotoxicity levels was determined with the colorometric MTT method [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], and the apoptosis level by Acridine Orange-Ethidium Bromide (AO-EB) double staining method. Results: When examining the results of both cytotoxicity and apoptosis, it was observed that the highest cytotoxic (87.57 ± 4.3%) and apoptotic (59.76 ± 3.57) effects were achieved with root extracts at the same concentrations (p <0.001). According to MTT results, IC50 value of root extracts was determined to be 61 µg/mL and cell death at 500 µg/mL as 91.92 ± 3.9. Conclusion: It has been concluded that Rheum ribes L. plant has anticancer activity on malignant melanoma cells and the root extract has a higher anti-cancer drug potential compared to the stem and barks of the plant.

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