Özet:

In the study, alterations in antibody titers, proinflammatory and antiinflammatory cytokine levels were determined in serum samples collected at various periods before and after administration of inactive IBR and iPPVO to cattle. It was aimed to investigate the immunomodulatory effects of inactivated parapoxvirus ovis (iPPVO) in cattle vaccinated with inactivated-IBR vaccine. In the study, 40 unvaccinated, clinically healthy cattle of different breeds aged older then 3 months were used. Three groups of cattle were formed as control group 1 (n=10), control group 2 (n=10) and experimental group (n=20). iPPVO was applied to the cattle in the control group 1 and experimental groups on the 0, 2nd, and 4th days. Blood samples were colleted from all the animals after 6th hours of the injections applied on 0th and 4th days of the study. Commercially available ELISA kits were used to determine serum levels of of IL-2, IL-6, IL-12 and IFN-γ. Furthermore, virus neutralization test was also performed to detect virus neutralizing antibody titres. In the present study, serum levels of IL-2, IL-6 IL-12, and IFN-γ levels were found to be significantly higher in the experimental group compared to that of the control group 1 and control group 2 (p<0,05). The differences between control group 1 and control group 2 groups were not statistically significant. In conclusion, iPPVO increased the levels of cytokines in IBR vaccinated cattle due to its immunomodulatory effects. In addition, virüs neutralizing antibody titers were found to be significantly higher in cattle that received vaccine and iPPVO.

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