Abstract:

Two commercial types of Hydro pericardium Hepatitis Syndrome (HHS) oily inactivated vaccines were evaluated. The first was (Angavac) French in origin (Merial Co.) the 2nd is (Nobilis Hepatitis) Holland in origin (Intervet Co.). A unified program of vaccination had been used for both vaccines. One ten days old chicks were vaccinated by subcutaneous intramuscular route of injection with both vaccines in each age separately . Serum antibodies titer weekly measured by using Indirect haemagglutination test (IHA) agar gel diffusion test. Challenge test at 4 week of age was done by virulent avian adeno virus serotype – 4 (VAAV-4) as well as virulent field isolate was used as challenge virus. The result showed highest antibodies titer at week age. Angavac vaccine gave highest titer than Nobilis Hepatitis vaccine IHA was more sensitive than AGDT. All groups vaccinated with Angavac gave 100% protection in challenge test with (VAAV-4) at 28 days. While in Holland vaccine the protection ratio was 60-80% only, but the ratio of protection of both vaccines did not exceed than 40-50% when the field isolate was used as challenge virus.

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