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Sunulan çalışmanın amacı kemik dolgu malzemesi ve ilaç taşıma sistemi olarak kullanılabilecek kitosan mikro kürelerin üretimi, karakterizasyonu ve performansının in vitro olarak belirlenmesidir. Bu amaçla; kitosan esaslı mikro küreler emülsiyon çarpraz bağlama yöntemi ile üretilmiş olup, mikro kürelerin boyut, şekil ve ilaç yükleme verimliliklerine etki eden karıştırıcı hızı, çapraz bağlama ajanı gibi faktörler incelenmiştir. İlaç çalışmalarında kullanılmak üzere bakteriyel enfeksiyonların tedavisinde kullanılan antibiyotik ciprofloxacin tercih edilmiştir. Ayrıca, hazırlanan mikro kürelerin biyoaktivitelerini test etmek için yapay vücut sıvısı (SBF) hazırlanmış ve 3 hafta boyunca mikro küreler SBF içerisinde bekletilerek hidroksiapatit çökelmesi (HA) takip edilmiştir. SBF içerisine konulan mikro küreler haftalık olarak toplanmış, Taramalı Elektron Mikroskopu (SEM) ve Fourier Dönüşümlü Kızılötesi Spektroskopisi (FTIR) ile analiz edilmiştir. Işık Mikroskopu ve SEM analizlerine göre küre boyutları karıştırma hızı arttıkça azalmış ve 102,27 ± 34,58 µm den 16,38 ± 3,26 µm ye kadar düşmüştür. Düşük miktarda kullanılan çapraz bağlayacı ajan glutaraldehit (GA) (NH2:CHO, 10:1 mol:mol) ise küre şekillerinde bozukluğa neden olurken, yüksek miktarda GA kullanımı (NH2:CHO, 1:10 mol:mol) kürelerin şeklinde iyileşmeye, küre boyutunda ve ilaç yükleme verimliliğininde azalmaya, ilaç salım hızında ise yavaşlamaya neden olmuştur. SBF ile inkübe edilen mikro kürelerin SEM görüntülerine göre HA yapıları 1. haftadan sonra yüzeylerde birikmeye başlamış, bu yapılar 3. haftada yüzeylerde daha belirgin hale gelmiştir. FTIR analizleri de HA çökelmesini işaret eden PO4-3 gruplarının 3. haftada daha belirgin olduğunu göstermiştir. Bu sonuçlara göre antibiyotik ciprofloxacin yüklü kitosan mikro küreler emülsiyon çapraz bağlama yöntemi ile başarılı bir şekilde üretilmiş olup, karıştırma hızı ve çapraz bağlayıcı ajanı GA miktarlarının mikro küre boyut, şekil ve salım profillerine etkisi ortaya konmuştur. Biyoktivite testlerinde kitosan mikro kürelerin, osteoentegrasyonu arttırma potansiyelinin olduğu gösterilmiştir. Dolayısıyla kitosan mikro küreler, kemik doku hasarlarında lokal olarak biyomoleküllerin salımını yapabilen dolgu malzemesi olarak veya implant malzemelerin yüzeylerinin modifikasyonunda kullanılabilir.
The aim of the presented study is to determine in vitro the production, characterization and performance of the kitosan micro curves that can be used as bone filling material and medicinal transportation system. For this purpose, microcircles based on kitosan are manufactured using the emulation cross-connection method, and factors such as the mixing speed, cross-connection agent, which influence the size, shape and drug charging efficiency of microcircles have been studied. The antibiotic ciprofloxacin used in the treatment of bacterial infections for use in drug studies was preferred. In addition, artificial body fluid (SBF) was prepared to test the bioactivity of prepared micro curves, and the micro curves were kept within SBF for 3 weeks, followed by hydroxyapathy disintegration (HA). The micro curves placed in the SBF were collected weekly, analyzed with the Scream Electron Microscope (SEM) and the Fourier Convertible Infrared Spectroscopy (FTIR). According to the Light Microscope and SEM analysis, the globe dimensions increased the mixing speed and decreased from 102.27 ± 34,58 μm to 16.38 ± 3.26 μm. The low amount of cross-binding agent glutaraldehyde (GA) (NH2:CHO, 10:1 mol:mol) has caused disturbance in the globe forms, while the high amount of use of GA (NH2:CHO, 1:10 mol:mol) has caused healing in the globe forms, decreased in the globe size and drug load efficiency, and slowed in the release rate. According to the SEM images of micro curves incubated with SBF, the HA structures began to accumulate on the surface after the 1st week, these structures 3. It has become clearer on the surface over the week. FTIR analyses also showed that the PO4-3 groups that indicate HA collapse were more noticeable in the 3rd week. According to these findings, the antibiotic ciprofloxacin loaded kitosan micro curls were successfully produced with the emulation cross-binding method, and the impact of the mixing speed and quantities of the cross-binding agent GA on the micro-cloud size, shape and release profiles has been shown. In the bioactivity tests, it has been shown that microcirculations in kitosan have the potential to increase osteointegration. Therefore, the microcircles can be used as a filling material that can locally release biomolecules in bone tissue damage or in the modification of the surfaces of implant materials.
The aim of the present study is to synthesize, characterize chitosan microspheres that can be used as bone-filling material and drug carrier system, and to evaluate its performance in vitro. For this purpose; chitosan-based microspheres were synthesized by the emulsion cross-linking method and factors such as stirring rate and cross-linker that may affect the size, shape, and drug loading efficiency of microspheres were examined. Antibiotic ciprofloxacin that is used in the treatment of bacterial infection was preferred for drug studies. Furthermore, simulated body fluid (SBF) was prepared to determine the bioactivity of the microspheres, and hydroxyapatite (HA) precipitation on microspheres was followed for 3 weeks in SBF. Microspheres were collected from SBF weekly and characterized using light microscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR). According to the light microscope and SEM analysis, the sphere dimensions decreased as the stirring rate increased and decreased from 102,27 ± 34,58 µm to 16,38 ± 3,26 µm. Low amount of cross-linking agent ( glutaraldehyde (GA)), (NH2: CHO, 10: 1 mol: mol) caused distortion in the shape of microspheres, while the high amount of GA (NH2: CHO, 1:10 mol: mol) caused smooth microspheres, a reduction in the size and drug loading efficiency, and a slowdown in the drug release rate. According to the SEM images of the microspheres incubated with SBF, HA structures began to precipitate on the surfaces after the first week, and these structures became more pronounced in the third week. PO4-3 groups attributed to HA precipitation in FTIR spectrum were more obvious at week 3. In conclusion; antibiotic ciprofloxacin loaded chitosan microspheres were synthesized successfully by the emulsion crosslinking method, and the effect of stirring rate, crosslinking agent GA amounts on microsphere size, shape, and release profiles were demonstrated. Enhanced HA precipitation was shown on chitosan microspheres in bioactivity tests. Hence, chitosan microspheres may be used as bone-filling material that can release biomolecules to damaged sites locally, or as coating to modify the surfaces of implant materials.
Alan : Fen Bilimleri ve Matematik; Mühendislik
Dergi Türü : Uluslararası
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