Özet:

Aims: The aim of this study is to investigate the anti-cancer and anti-proliferative effect of spider web extract, and mitochondrial apoptosis signaling response via gene expression levels belonging to caspase-3 depended apoptosis in both healthy Astrocyte and cancerous HeLa cell lines. Methods: We used HeLa and C8-D1A Astrocyte type I clone cell lines as the human cervical cancer and healthy cell line, respectively. We treated the cells with spider web extract and performed MTT assay. IC50 was calculated by probit analysis. Molecular fluorescence staining and TALI cytometer measurements were also applied. The response of the mitochondrial apoptosis pathway to spider web extract treatment was analyzed by the qRT-PCR assay. Results: The spider web extract administered in different concentrations to the HeLa cell line reduced cell viability at a statistically significant level after 24 hours of application. In the astrocyte cell line, the spider web extract did not cause statistically significant cell death. In the analysis of gene expressions, a strong mitochondrial apoptosis signaling was observed. Conclusion: While spider web extract does not cause any cytotoxic effects on healthy cell lines, it causes a strong fatal effect in cancer cell line. In addition, IC50 dose of the spider web extract is satisfying compared to many other natural products in the market which have anti-cancer effects. Spider web extract causes a programmed cell death by following death signaling pathways.  

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