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Kedi ve Köpeklerden İzole Edilen Staphylococcus Türlerinde Çoklu İlaç Dirençliliğinin Araştırılması
2020
Journal:  
Veterinary Sciences and Practices
Author:  
Abstract:

Kedi ve köpeklerden sıklıkla izole edilen Staphylococcus türlerinin metisilin ve çoklu ilaç dirençliliği (MDR) artışı  çalışmamızın temelini oluşturdu. Bu amaçla, Yakın Doğu Üniversitesi Hayvan Hastanesi’ne getirilen 100 adet köpek ve 42 adet  kediye ait toplamda 142 adet klinik örnek (yara, kulak, göz, burun, deri svapları gibi) çalışıldı. İncelenen örneklerden izole  edilen Staphylococcus spp. izolatlarının tür düzeyinde identifikasyonu, VITEK-2 mikrobiyal ID/ADT test sistemi ve API Staph  identifikasyon sistemi ile gerçekleştirildi. Staphylococcus izolatlarının antimikrobiyal duyarlılık analizleri VITEK-2 otomatize  sistem aracılığı ile gerçekleştirildi. İncelenen numunelerden 78 adet (%54.92) Staphylococcus spp. izole edildi. İzolatların 52’si  (%66.67) Koagulaz pozitif Staphylococcus (CoPS) ve 16’sı (%20.51) Koagulaz negatif Staphylococcus (CoNS) olarak identifiye  edildi. CoPS’lar arasında en yüksek oranda identifiye edilen türlerin Staphylococcus (S.) pseudintermedius (%38,46) ve S.  aureus (%25.64); CoNS’lar arasında ise S. chromogenes (%10.26) olduğu belirlendi. Antimikrobiyal madde duyarlılık analizleri  sonucu, 11 (%28.2) izolat metisilin dirençli S. aureus (MRSA), 10 (%25.64) izolat metisilin dirençli S. pseudintermedius (MRSP)  ve 10 (%25.64) izolat metisilin dirençli Koagulaz negatif Staphylococcus (MRCoNS) olarak belirlendi. Ayrıca çoklu ilaç direnci  tespit edilen türlerin 6’sında (%40.00) MDR-MRSP ve 4’ünde (%26.66) MDR-MRSA pozitifliği belirlendi. Toplamda 23 adet  MDR Staphylococcus spp. izolatının 21 adedi köpeklerden ve 2 adedi de kedilerden izole edilmiştir. Çalışmada elde edilen  veriler ışığında artan çoklu ilaç direncinin güncel yaklaşımlarını hayvanlardan izole edilen Staphylococcus türlerinde  inceleyerek tedavi protokollerinin bu çerçevede uygulanmasına katkı sağlandı.

Keywords:

Study of Multiple Drug Resistance in Species of Staphylococcus Isolated from Cats and Dogs
2020
Author:  
Abstract:

The increase in methysillin and multi-drug resistance (MDR) of staphylococcus species, often isolated from cats and dogs, formed the foundation of our study. For this purpose, a total of 142 clinical samples of 100 dogs and 42 cats (such as wounds, ears, eyes, nose, skin spots) were studied. Staphylococcus spp is isolated from the examined samples. The identification at the type level of the isolates was carried out with the VITEK-2 microbial ID/ADT test system and the API Staph identification system. Antimicrobial sensitivity analysis of Staphylococcus islands was carried out through the VITEK-2 automated system. 78 amounts (54.92) of the examined samples are Staphylococcus spp. It was isolated. 52 islands (66 per cent) 67) Coagulase positive Staphylococcus (CoPS) and 16 (%20.51) Coagulase negative Staphylococcus (CoNS) were identified. The highest number of species identified among CoPSs was Staphylococcus (S.) pseudintermedius (38.46) and S. aureus (25.64); among CoNSs S. chromogenes (10.26) were identified. As a result of an antimicrobial substance sensitivity analysis, 11 (28.2%) is isolated methylococcus resistant S. aureus (MRSA), 10 (25.64) is isolated methylococcus resistant S. pseudintermedius (MRSP) and 10 (25.64) is isolated methylococcus resistant Coagulase negative Staphylococcus (MRCoNS). Moreover, MDR-MRSP positivity was identified in 6 of the types identified with multi-medicine resistance (40.00%) and MDR-MRSA positivity in 4 (26.66) of the types identified with multi-medicine resistance. There are 23 MDR staphylococcus spp. It is isolated from 21 dogs and 2 cats. In light of the data obtained in the study, the study of current approaches to increasing multi-drug resistance in species of Staphylococcus isolated from animals contributed to the implementation of treatment protocols in this framework.

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Veterinary Sciences and Practices

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