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Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en sık rastlanan üçüncü kanser türüdür. Her yaş grubunda görülebildiği gibi kalıtımsal kökenli değildir. Bu veriler göz önüne alındığında kanserin tedavisi için yeni ilaç kombinasyonlarının araştırılması önemli bir ihtiyaçtır. Bu çalışmada, anti-kanser aktivitesi bilinmeyen Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon karayosunu türlerinden elde edilen ekstraktlarının HCT116 kolon kanser hücre hatlarına karşı etkileri değerlendirilmiştir. Karayosunu türlerinin etil alkol çözücüsünde elde edilen ekstrelerinden, DMSO ile stok solüsyonlar hazırlanarak, çeşitli konsantrasyonlarda sitotoksite test (MTT) deneyleri yapılmıştır. HCT116 serisinde bulunan tüm karayosunu türlerinin IC50 değerlerine göre belirlenen dozlar aynı şekilde mezenkimal kök hücrelere (MKH) uygulanmıştır. Ayrıca, Fourier transform kızılötesi (FTIR) spektroskopisi üç karayosununa maruz kalan kanser hücrelerini analiz etmek için kullanılmıştır. Sonuç olarak, karayosunu türlerinin HCT116 kolorektal kanser hücre hattına karşı yüksek oranda antiproliferatif etki gösterdiği, bunun aksine MKH hücrelerinde letal olmadığı tespit edilmiştir. Ayrıca, FTIR spektrumu ile elde edilen sonuçların tüm hücre proliferasyonu verileriyle paralel seyrettiği moleküler düzeyde de belirlenmiştir. Tüm sonuçların ışığında bu karayosunu türlerinin, biyoaktif bileşenleri belirlendiği takdirde, potansiyel kanser ilacı olma özelliğine sahip olduğu düşünülmektedir.
Colon cancer is the third most common type of cancer in the world, with more than one million new diagnoses revealed every year. It is not hereditary as it can be seen in every age group. Given these data, the research of new medication combinations for the treatment of cancer is an important need. In this study, the anti-cancer activity is unknown Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon caraious species have evaluated the effects of the extracts obtained against the HCT116 colon cancer cell lines. From the extracts obtained in the ethyl alcohol solver of the Karayosunu species, preparing stock solutions with DMSO, cytotoxity test (MTT) tests in various concentrations have been conducted. The doses determined according to the IC50 values of all HCT116 species are applied in the same way to mesenchimal root cells (MKH). Furier transform infrared spectroscopy (FTIR) has also been used to analyze cancer cells exposed to three caraios. As a result, it has been found that the species of karayosunu have a high rate of antiproliferative effect against the HCT116 colorectal cancer cell line, but it is not fatal in the MKH cells. It is also determined at a molecular level where the results obtained by the FTIR spectrum are seen parallel to the data of all cell proliferation. In light of all the results, it is believed that these kinds of caraiosus, if the bioactive components are identified, have the ability to be a potential cancer drug.
Colon cancer is the third most common type of cancer in the world, with more than a million new diagnoses every year. As can be seen in all age groups, it is not inherited. Considering these data, it is an important need to research new drug combinations for the treatment of cancer. In this study, the effects of extracts obtained from Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon bryophyte species with unknown anticancer activity against HCT116 colon cancer cell lines were evaluated. Cytotoxicity test (MTT) experiments were performed at various concentrations by preparing stock solutions with DMSO from extracts of moss species obtained in ethyl alcohol solvent. The doses determined according to the IC50 values of all moss species in the HCT116 series were applied to the mesenchymal stem cells (MSCs) in the same way. In addition, Fourier transform infrared (FTIR) spectroscopy was used to analyze cancer cells exposed to three mosses. As a result, it was determined that moss species showed a high antiproliferative effect against HCT116 colorectal cancer cell line, but on the contrary, it was not lethal in MSC cells. In addition, it was determined at the molecular level that the results obtained with the FTIR spectrum were parallel to the cell proliferation data. In the light of all the results, these Bryophyte species are considered to be potential cancer drugs if their bioactive components are determined.
Field : Fen Bilimleri ve Matematik
Journal Type : Uluslararası
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