Abstract:

The purpose of this study is to research on fruit flies in Mekong Delta region in Vietnam, differences and similarities among characteristics of B.dorsalis, B.correcta, esp. B.carambolae, B.cucurbitae and B. tau species and then give some recommendations. The precise identification’s procedure of fruit flies species is important for the management of fruit fly pests to enhance quarantine services on fruit and vegetable. In this study, we collected 15 samples from guava and bitter gourd fruits in 3 provinces belong to the Mekong Delta region. From that, a nucleotide sequences of 700 bp of the cytochrome oxidase subunit II (COII) gene of mitochondria DNA (mtDNA 13 and mtDNA 20) was amplified by PCR with the mtD13 and mtD20 primer pairs using DNA of 15 specimens from fruit flies samples. Based on the target gene sequence polymorphism (488 bp) analysis on mitochondrial COII, 15 fruit fly samples were identified as belonging to two subgenera Bactrocera and Dacus, sequences of a 700 bp portion of the cytochrome oxidase subunit II of the 15 specimens from collected fruit flies, the results showed that they belong to the genus Bactrocera (12 guava fruit fly samples belong to the subgenus Bactrocera and 3 bitter gourd samples belong to the subgenus Zeugodacus (or Dacus)) with phylogenetic tree supported by high bootstrap value as (97.54-98.02%). Two genera had been identified by 2 haplotype with 11 different mutations on the DNA sequences. Genus Bactrocera has three different species named B. dorsalis, B. correcta and B. carambolae with the homogenus sequences come up to 98.02%. In genus Zeugodacus, DNA sequences of the two species named Bactrocera curcubitae and B. tau was high heterologous with each other (99-100%) with 15 different mutations on their DNA sequences. The DNA sequences of the species belong to the genus Zeugodacus have high homologus (98.91-99.39%).

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