Özet:

This study was aimed at investigating the effect of Esculetin on liver and kidney damage created in rates with methotrexate (MTX). The study consisted of a total of 4 groups, including Group 1 (Control Group), Group 2 (MTX), Group 3 (Eskuletin) and Group 4 (MTX+Eskuletin). For rats in groups 3 and 4 the dose of 100 mg/kg was administered for 14 days. In addition, MTX was intraperitonally injected at a dose of 20 mg/kg on the 9th day of the trial to rats in Group 2 and 4. The work was terminated 5 days after the MTX application. At the end of the study, it was found that the levels of liver and kidney tissue malondaldehyde (MDA) in the MTX group were increased, the levels of reduced glutathion (GSH) in the liver were reduced, the activity of glutathion peroxidase (GSH-Px) and catalase (CAT) enzymes in both tissues was significantly reduced, and the application of MTX caused oxidative stress in these tissues. In groups treated with esculetin, the levels of MDA in the liver and kidney tissue were reduced, GSH-Px and CAT activity increased. Additionally, the application of the sculptus has also been shown to reduce the levels of transaminase (ALT) and aspartate transaminase (AST) in increased serum area. In histopathological analyses, MTX was found to cause degenerative and fire changes such as hydropic degeneration in both the liver and the kidneys, fire cell infiltration; the application of the eskulet was found to significantly reduce these damage. As a result, it was determined that the eskuletine, with its antioxidant and anti-inflammatory effects, prevents or minimizes the damage to the liver and kidney caused by MTX.

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