Abstract:

In this study, the in vitro effects of some metal ions (Ag+, Cd2+, Cu2+, Fe2+, Ni2+ and Pb2+) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag+, Cd2+, Cu2+, Ni2+ and Pb2+ inhibited fish liver G6PD; Ag+, Cd2+, Fe2+, Ni2+ and Pb2+ inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+. Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.

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